Recombinant Salmonella typhi fliC Antigen, His tagged

• Cat.No.: flag-021S
• Product Overview: Some of the antigenic regions of the Salmonella typhi 52 kDa flagellin protein produced by the fliC gene, have been prepared as a recombinant antigen fused to a his-tag in its N-terminus.

Specification

• Species: Salmonella typhi
• Source: E.coli
• Tag: His
• Description: flagellin is the flagella antigen, d, of Salmonella typhi, which is present as a phase-l antigen, and corresponds to the 52 kDa-flagellin antigen. Salmonella serotypes usually alternated between the production of the antigenic forms of flagella, termed "phase-I" and "phase-2". Some species, including Salmonella typhi are monophasic, with only a phase-l antigen.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 35-25 kDa, while relative molecular mass calculated from amino acid sequence is 20,920.96 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 50 mM MES pH 6.5, 0.1 M KCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.24 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Sukosol T, Sarasombath S, Mongkolsuk S, Songsivilai S, Chaiyaroj S, Pongsunk S, Ekpo P. Molecular cloning and expression of Salmonella typhi flagellin: characterization of 52 kDa specific antigen of S. typhi. Asian Pac J Allergy Immunol 1993; 11: 57-69.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.