Recombinant S. pyogenes Cas9 Protein, His-tagged

• Cat.No.: Cas9-06S
• Product Overview: Recombinant full length Cas9-NLS from S. pyogenes was expressed in E. coli using a C-terminal His tag. Cas9-NLS contains a nuclear localization sequence for optimal activity.

Specification

• Description: In bacteria and archaea CRISPR- Cas systems provide sequence based adaptive immunity against mobile genetic elements such as phages and plasmids. Cas9 is a multidomain nuclease from the bacterium S. pyogenes characteristic of type II CRISPR- Cas systems. Using single guide RNA (sgRNA), Cas9 is targeted to specific genomic loci where it generates a blunt ended double strand beak. Cas9 can be used for genetic engineering experiments such as genome editing and generating transgenic models.
• Source: E. coli
• Species: S. pyogenes
• Tag: His
• Molecular Mass: Observed MW: ~160 kDa
  Calculated MW: ~161 kDa
• Purity: >70%
• Stability: One year at -70 centigrade from deta of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.
• Concentration: 0.1 μg/μL
  6.26 pmol/μg
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.
• Shipping: On dry ice.
• Full Length: Full L.

Gene Information

• Official Symbol: Cas9
• Synonyms: Csn1; Csx12; Spy_1046; SpyCas9
• Protein Refseq: AAK33936
• UniProt ID: Q99ZW2