Active Recombinant Streptococcus pyogenes M1 cas9 Protein, His-tagged

• Cat.No.: cas9-12S
• Product Overview: A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Streptococcus pyogenes M1
• Tag: His
• Predicted N Terminal: Met
• Form: Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.
• Bio-activity: 1 ug = 5 U
  1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37°C
• Molecular Mass: The recombinant s. pyogenes serotype M1 cas9 consists of 1407 amino acids and predicts a molecular mass of 163 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: Liquid. It is shipped out with blue ice.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.