Recombinant Rhesus TNF protein

• Cat.No.: TNF-1387R
• Product Overview: Recombinant Rhesus TNF protein was expressed in Escherichia coli.

Specification

• Species: Rhesus macaque
• Source: E.coli
• Tag: Non
• Protein Length: 157
• Description: Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Rhesus macaque and human TNF-α show approximately 98 % homology at the amino acid level. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg in the presence of actinomycin D.
• Molecular Mass: Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
• AA Sequence: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELTDNQLVVPSEGLYLIYSQVLFKGQGCPSNHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINLPDYLDFAESGQVYFGIIAL
• Endotoxin: Less than 1 EU/μg of rRhTNF-α as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: TNF
• Official Symbol: TNF
• Synonyms: TNFSF2, Cachectin, Differentiation-inducing Factor, DIF, Necrosin, Cytotoxin
• Gene ID: 715467
• mRNA Refseq: NM_001047149.1
• Protein Refseq: NP_001040614.1
• UniProt ID: P48094