Recombinant Rhesus macaque VSIR protein, His-tagged

• Cat.No.: VSIR-363R
• Product Overview: Rhesus macaque B7-H5, His tag is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Thr 192 (Accession # NP_001177807.1).

Specification

• Description: Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
• Source: HEK293
• Species: Rhesus macaque
• Tag: His
• Predicted N Terminal: Phe 33
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Phe 33 - Thr 192
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Storage buffer: PBS, pH7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: VSIR V-set immunoregulatory receptor [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: VSIR
• Synonyms: C9orf54; C9H10orf54; platelet receptor Gi24
• Gene ID: 709595
• mRNA Refseq: NM_001190878.1
• Protein Refseq: NP_001177807.1
• UniProt ID: F7F6S6