Recombinant Human VSIR Protein, His-Avi-tagged, Biotinylated

• Cat.No.: VSIR-838H
• Product Overview: Biotinylated Human B7-H5 is expressed from HEK293 cells. It contains AA Phe 33 - Ala 194 (Accession # AAH20568.1). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 33-194 a.a.
• Predicted N Terminal: Phe 33
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 20.8 kDa. As a result of glycosylation, the protein migrates as 35-45 kDa under reducing condition, and 35-45 kDa under non-reducing condition (SDS-PAGE).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: VSIR
• Synonyms: B7-H5, SISP1, Gi24, VISTA