Recombinant Rhesus macaque DLL3 protein, His-tagged

• Cat.No.: DLL3-171R
• Product Overview: Recombinant Rhesus macaque DLL3 protein(F6TFV4)(Ala27-Arg490), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: Ala27-Arg490
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 50mM NaCl, 200mM L-arginine (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rhesus macaque DLL3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 51 kDa. Due to glycosylation,the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.