Recombinant Rhesus CD209 protein, His-tagged

• Cat.No.: CD209-727R
• Product Overview: Recombinant Rhesus CD209(Lys62-Glu381) fused with the C-terminal His tagg was expressed in HEK293.

Specification

• Description: Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.
• Source: HEK293
• Species: Rhesus
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant rhesus CD209 consists of 339 amino acids and predicts a molecular mass of 38.8 kDa.
• Protein length: Lys62-Glu381
• Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigradeto -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: CD209 CD209 molecule [ Macaca mulatta ]
• Official Symbol: CD209
• Synonyms: DC-SIGN; CD209 antigen; DC-SIGN1; dendritic cell-specific ICAM-3 grabbing non-integrin; dendritic cell-specific ICAM-3-grabbing non-integrin 1; putative mannose-binding C-type lectin; putative mannose-binding C-type lectin mDC-SIGN
• Gene ID: 574211
• mRNA Refseq: NM_001032870
• Protein Refseq: NP_001028042
• UniProt ID: Q95J96
• Chromosome Location: chromosome: 19
• Pathway: Measles, organism-specific biosystem; Phagosome, conserved biosystem; Tuberculosis, conserved biosystem