Recombinant Rat Trace-amine-associated Receptor 1, His-tagged

• Cat.No.: Taar1-1285R
• Product Overview: Recombinant Rat Receptor Associated Protein produced inE.Coliis a single, non-glycosylated polypeptide chain having a molecular mass of 38,862 Dalton. The Recombinant RAP Rat contains 6xHis tag and 1xC-myc, RAP Rat is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Taar1-1285R
• Description: Receptor-associated Protein (aka RAP) averts the GCM-mediated enhancement of axon growth- ApoE-containing lipoproteins which are known to bind to receptors of the LDLr superfamily. In the presence of RAP, GCM doesn't increase the axon extension rate in relation to base medium. Furthermore, the addition of RAP to the base medium doesn't change the rate of axon extension. This implies that the growth stimulatory effect of apoE-containing lipoproteins secreted by glial cells is mediated by the LDLr family receptors. RAP annuls the growth stimulatory effect of GCM.
• Source: Escherichia Coli.
• Special Note: Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10mM EDTA. Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: The protein (1mg/ml) was lyophilized after from a sterile solution containing TBS pH-7.5, 0.1% BSA and 0.09% NaN3.
• Solubility: It is recommended to reconstitute the lyophilized RAP Rat in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Stability: Lyophilized RAP Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RAP Rat should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Taar1 trace-amine-associated receptor 1 [ Rattus norvegicus ]
• Synonyms: Taar1; trace-amine-associated receptor 1; Tar1; Trar1; trace amine associated receptor 1; trace amine receptor 1
• Gene ID: 113914
• mRNA Refseq: NM_134328
• Protein Refseq: NP_599155
• UniProt ID: Q923Y9
• Pathway: Neuroactive ligand-receptor interaction
• Function: G-protein coupled amine receptor activity; receptor activity; trace-amine receptor activity