Active Recombinant Human TNF Protein, FLAG-tagged

• Cat.No.: TNF-408H
• Product Overview: Recombinant protein of human TNF with tracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 85-233;18-111 a.a.
• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
• Form: Lyophilized
• Bio-activity: Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10 ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
• Molecular Mass: ~34kDa (SDS-PAGE)
• Endotoxin: <0.02 EU/μg purified protein (LAL test; Lonza).
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Storage: Short Term Storage: 4 centigrade.
  Long Term Storage: -20 centigrade.
• Concentration: 0.1 mg/mL after reconstitution.
• Storage Buffer: PBS
• Reconstitution: Reconstitute with 100 μL sterile water.
• Handling: After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homo sapiens (human) ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375