Active Recombinant Human SOST, His-tagged

• Cat.No.: SOST-925H
• Product Overview: Recombinant humanSOST with an N­terminal 7­His tag was expressed in mouse myeloma cell, 22.5 kDa.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Description: Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
• N­terminal Sequence Analysis: His
• Activity: Measured by its ability to inhibit Wnt­3a­induced alkaline phosphatase production by MC3T3-E1mouse preosteoblast cells. The ED50 for this effect is typically 1.5-6μg/mL in the presence of 3ng/mL Recombinant Mouse Wnt­3a. Measured by its ability to inhibit BMP­6­induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. 30µg/mL of rhSOST will antagonize hBMP­6 induction of alkaline phosphatase in MC3T3E1 cells by 50­80%.
• Structure / Form: Monomer
• Predicted MolecularMass: 22.5 kDa
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method
• Purity: > 95% as determined by SDS-PAGE.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°Cas supplied. 1 month, 2 to 8°Cunder sterile conditions after reconstitution. 3 months, -20 to -70°Cunder sterile conditions after reconstitution.

Gene Information

• Gene Name: SOST sclerosteosis [Homo sapiens]
• Official Symbol: SOST
• Synonyms: SOST; VBCH; sclerostin; sclerosteosis
• Gene ID: 50964
• mRNA Refseq: NM_025237
• Protein Refseq: NP_079513
• MIM: 605740
• UniProt ID: Q9BQB4
• Chromosome Location: 17q11.2
• Function: heparin binding; protein binding; transcription factor binding