Recombinant Rat Protein Kinase, cGMP-dependent, Type II

• Cat.No.: Prkg2-603R
• Product Overview: Recombinant Rat Prkg2 was produced inSf9cells. MW = 86kDa; monomer.

Specification

• Species: Rat
• Source: Sf9 Cells
• Tag: Non
• Description: PKG are serine/threonine kinases that are present in a variety of eukaryotes ranging from the unicellular organism Paramecium to humans. Two PKG genes, coding for PKG type I (PKG-I) and type II (PKG-II), have been identified in mammals. The N-terminus of PKG-I is encoded by two alternatively spliced exons that specify for the PKG-Iα and PKG-Iβ isoforms. PKG-Iβ is activated at ~10-fold higher cGMP concentrations than PKG-Iα. The PKG-I and PKG-II are homodimers of two identical subunits (~75 kDa and ~85 kDa, respectively) and share common structural features.
• Purity: ≥90% (SDS-PAGE).
• Formulation: Liquid. In 20mM TRIS, pH 7.4, containing 1mM EDTA, 5mM β-mercaptoethanol, 100mM NaCl, 10U/ml trasylol, 0.015% NP40 and 50% glycerol.
• Concentration: 50µg/ml.
• Specific Activity: ~0.5-2.0U/mg protein. 5-fold activation by cGMP (5µM). One unit corresponds to 1µmol/mg/min using VASPtide (RRKVSKQE) as substrate, under the following optimal conditions: 10mM HEPES (pH 7.4), 5mM MgCl2, 1mM DTE, 0.2mM EDTA.
• Stability: The enzyme is relatively unstable. To prevent loss of activity when performing experiments, keep the stock vial of the enzyme on ice (0-4°C) at all times. The enzyme loses activity during a single freeze/thaw cycle. To avoid adherence of the product to plastic, the use of siliconized vials and tips is recommended. After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Prkg2 protein kinase, cGMP-dependent, type II [ Rattus norvegicus ]
• Synonyms: Prkg2; protein kinase, cGMP-dependent, type II; cGKII; GDPKII; cGMP-dependent protein kinase 2; cGK2; CGK 2; cGMP-dependent protein kinase II; cGMP dependent protein kinase type II; protein kinase cGMP- dependent type 2; type II cGMP-dependent protein kinase; protein kinase, cGMP- dependent, type 2; protein kinase, cGMP- dependent, type II; EC 2.7.11.12
• Gene ID: 25523
• mRNA Refseq: NM_013012
• Protein Refseq: NP_037144
• UniProt ID: Q64595
• Chromosome Location: 14p22
• Pathway: Gap junction; Long-term depression; Olfactory transduction
• Function: ATP binding; cGMP binding; cGMP-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity; transferase activity