Recombinant Rat Pkm protein, His & T7-tagged

• Cat.No.: Pkm-2615R
• Product Overview: Recombinant Rat Pkm aa. (Val324~Pro531 (Accession # P11980)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: M1 and M2 isozymes of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis.
• Source: E. coli
• Species: Rat
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.4kDa
• Protein length: Val324~Pro531
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: Pkm pyruvate kinase, muscle [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Pkm
• Synonyms: Pkm; pyruvate kinase, muscle; PK; Pk3; Pkm2; PKM12; pyruvate kinase PKM; M2 pyruvate kinase; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme
• Gene ID: 25630
• mRNA Refseq: NM_053297.2
• Protein Refseq: NP_445749.1
• UniProt ID: P11980