Active Recombinant Human PKM2 Protein (1-531aa), N-6×His-tagged

• Cat.No.: PKM2-11H
• Product Overview: Recombinant human PKM2 protein (1-531aa) fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity: > 25,000 pmol/min/μg. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37 centigrade.
• Molecular Mass: 60.1 kDa (551aa)
• Protein length: 1-531
• AA Sequence: MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Gene Information

• Gene Name: PKM2 pyruvate kinase, muscle [ Homo sapiens (human) ]
• Official Symbol: PKM2
• Synonyms: PKM2; pyruvate kinase, muscle; pyruvate kinase isozymes M1/M2; OIP3; PK3; THBP1; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic; PKM; TCB; CTHBP; MGC3932
• Gene ID: 5315
• mRNA Refseq: NM_002654
• Protein Refseq: NP_002645
• MIM: 179050
• UniProt ID: P14618