Recombinant Rat P4ha1 protein, His & T7-tagged

• Cat.No.: P4ha1-1177R
• Product Overview: Recombinant Rat P4ha1 aa. (Val205~Glu534 (Accession # P54001) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Catalyzes the hydroxylation of -X-Pro-Gly- triplets to form hydroxyproline.
• Source: E. coli
• Species: Rat
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.3kDa.
• Protein length: Val205~Glu534
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 7.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: P4ha1 prolyl 4-hydroxylase subunit alpha 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: P4ha1
• Synonyms: P4ha1; prolyl 4-hydroxylase subunit alpha 1; PHalphaI; prolyl 4-hydroxylase subunit alpha-1; 4-PH alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; prolyl 4-hydroxylase alpha subunit; prolyl 4-hydroxylase, alpha polypeptide I
• Gene ID: 64475
• mRNA Refseq: NM_172062.2
• Protein Refseq: NP_742059.2
• UniProt ID: P54001