Recombinant Rat Ntn1 protein, His/GST-tagged

• Cat.No.: Ntn1-257R
• Product Overview: Recombinant Rat Ntn1(Pro313~Pro565) fused with two N-terminal Tags, His-tag and GST-tag was expressed in E. coli.

Specification

• Species: Rat
• Source: E.coli
• Tag: GST&His
• Protein Length: Pro313~Pro565
• Description: Ntn1 played an important role in many functions.
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Molecular Mass: 60.5kDa
• AA Sequence: PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSMEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIAXKCPKIK PLKKYLLLGN AEDSP
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Publications:
  Netrin-1 Improves Functional Recovery through Autophagy Regulation by Activating the AMPK/mTOR Signaling Pathway in Rats with Spinal Cord Injury (2017)
  Neuroprotection of netrin-1 on neurological recovery via Wnt/β-catenin signaling pathway after spinal cord injury (2020)
  The Role of Netrin-1 in Improving Functional Recovery through Autophagy Stimulation Following Spinal Cord Injury in Rats (2017)

Gene Information

• Gene Name: Ntn1 netrin 1 [ Rattus norvegicus ]
• Official Symbol: Ntn1
• Synonyms: NTN1; netrin 1; netrin-1
• Gene ID: 114523
• mRNA Refseq: NM_053731
• Protein Refseq: NP_446183
• Chromosome Location: 10q24
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; DCC mediated attractive signaling, organism-specific biosystem