Recombinant Rat myelin oligodendrocyte glycoprotein Protein, His tagged

• Cat.No.: MOG-043R
• Product Overview: The sequence (Accession #CAE84068) corresponding to the extracellular domain of rat MOG along with a 6×His tag was expressed in E. coli. The recombinant rat MOG (R-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC).

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: 1-125aa
• Description: The product of this gene is a membrane protein expressed on the oligodendrocyte cell surface and the outermost surface of myelin sheaths. Due to this localization, it is a primary target antigen involved in immune-mediated demyelination. This protein may be involved in completion and maintenance of the myelin sheath and in cell-cell communication. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Tag: 6×His
• Form: Solution
• Molecular Mass: 14.2 kDa
• Bio-activity: Female DA rats and Lewis rats (7-9 weeks old) were immunized (tail base s.c.) with 50-75 μg/animal of rat rMOG in complete Freund's adjuvant. DA and Lewis rats showed EAE symptoms such as limp tail, hind limb weakness, hind limb paralysis, and weight loss after induction. Please note that no other EAE induction protocols were tested including IFA/cytokine model.
• Resuspension condition: Rat MOG must be thoroughly mixed directly with Complete Freund's Adjuvant (CFA). Do not dilute recombinant rat MOG with buffers that have pH greater than 4.5. Protein will precipitate at pH higher than 4.5
• Endotoxin: Less than 0.1 EU per 1 μg of the protein as determined by Limulus Amebocyte Lysate (LAL) quantitative kinetic assay.
• Purity: ≥95% by SDS-PAGE
• Applications: ELISA; in vivo studies; Western Blotting
• Usage: Research use
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 1 mg/mL
• Storage Buffer: 25 mM sodium acetate buffer (pH=4.0)
• References: 1. Jayaram Bettadapura et.al. (1998) Journal of Neurochemistry 70 (4): 1593-1599
  2. Alfred R Oliver et al (2003) Journal of Immunology 171:462-468
  3. Hans-Christian Von Budingen et.al. (2001) Journal of Clinical Immunology 21 (3): 155-170
  4. Jerri-Anne Lyons et.al. (1999) European Journal of Immunology 29: 3432-3439
  5. Hans-Christian Von Budingen et.al. (2004) European Journal of Immunology 34: 2072-2083

Gene Information

• Gene Name: Mog myelin oligodendrocyte glycoprotein [ Rattus norvegicus ]
• Official Symbol: MOG
• Synonyms: MOG; myelin oligodendrocyte glycoprotein; myelin-oligodendrocyte glycoprotein; dystonia 2, torsion (autosomal recessive); MGC105427
• Gene ID: 24558
• mRNA Refseq: NM_022668
• Protein Refseq: NP_073159
• UniProt ID: Q63345