Recombinant Rat Musk Protein, mIgG2a tagged

• Cat.No.: Musk-44R
• Product Overview: Recombinant Rat Musk Protein (Glu22-Thr494) with Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Description: Enables protein kinase binding activity and protein tyrosine kinase activity. Involved in several processes, including positive regulation of macromolecule metabolic process; positive regulation of synaptic transmission; and sensory organ development. Located in cell projection; external side of plasma membrane; and neuromuscular junction. Human ortholog(s) of this gene implicated in congenital myasthenic syndrome 9 and fetal akinesia deformation sequence syndrome 1. Orthologous to human MUSK (muscle associated receptor tyrosine kinase).
• Source: Mouse myeloma cell line
• Species: Rat
• Tag: mIgG2a
• Protein length: 22-494aa
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. In the presence of Recombinant Rat Agrin, immobilized Recombinant Rat MuSK Fc Chimera (2 μg/mL at 100 μL/well) binds Recombinant Human LRP-4 with an ED50 of 0.06-0.3 μg/mL.
• Molecular Mass: 79 kDa
• Endotoxin: < 0.1 EU/μg by LAL.
• Purity: > 90 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• N-terminal Sequence Analysis: Glu22
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• References: 1. Valenzuela, D.M. et al. (1995) Neuron 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.

Gene Information

• Gene Name: Musk muscle, skeletal, receptor tyrosine kinase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Musk
• Synonyms: MUSK; muscle, skeletal, receptor tyrosine kinase; muscle, skeletal receptor tyrosine protein kinase; muscle-specific kinase receptor; muscle-specific tyrosine kinase receptor MuSK; muscle-specific tyrosine protein kinase receptor; muscle specific kinase (neural fold/somite kinase 1); Nsk1
• Gene ID: 81725
• mRNA Refseq: NM_031061
• Protein Refseq: NP_112323
• UniProt ID: Q62838