Recombinant Rat Mmp13 protein, His-tagged

• Cat.No.: Mmp13-506R
• Product Overview: Recombinant Rat Mmp13 (G3V742) aa. (Leu14~Cys466) fused with His tag was produced in HEK293F cells.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Leu14~Cys466
• Description: An enzyme that degrades collagen type I.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 53.4kDa; Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 97%
• Characteristic: Isoelectric point is 5.1.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Mmp13 matrix metallopeptidase 13 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Mmp13
• Synonyms: Mmp13; matrix metallopeptidase 13; collagenase 3; MMP-13; UMRCASE; interstitial collagenase; matrix metalloproteinase-13
• Gene ID: 171052
• mRNA Refseq: NM_133530.1
• Protein Refseq: NP_598214.1
• UniProt ID: G3V742