Recombinant Rat Klk1 protein, His-tagged

• Cat.No.: Klk1-5679R
• Product Overview: Recombinant Rat Klk1 aa. (Pro2~Glu86) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Pro2~Glu86
• Description: Serine protease; involved in processing of bioactive peptides.
• Form: Freeze-dried powder
• Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.3.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Klk1 kallikrein 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Klk1
• Synonyms: Klk1; kallikrein 1; Kal; KALA; Klk5; Klk7; Klk1l; Klna3; RGK-7; Klk1c7; RSKG-7; RATKALA; Kallikrein 1, renal/pancreas/salivary; esterase B; glandular kallikrein-7, submandibular/renal; kallikrein 1-like peptidase; kallikrein 1-related peptidase C7; kallikrein 5; kallikrein 7; kallikrein a3; kallikrein-related protein K1; proteinase A; renal kallikrein; tissue kallikrein
• Gene ID: 24523
• mRNA Refseq: NM_012593.1
• Protein Refseq: NP_036725.1
• UniProt ID: P36373