Active Recombinant Human KLK1 protein, His-tagged

• Cat.No.: KLK1-299H
• Product Overview: Recombinant Human KLK1 protein(NP_002248.1)(Met1-Ser262), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1,500 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Molecular Mass: The secreted recombinant human KLK1 is a monomeric protein after cleavage of the signal peptide. It consists of 251 amino acids and predictes a molecular mass of 28 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.
• Protein length: Met1-Ser262
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: KLK1 kallikrein 1 [ Homo sapiens ]
• Official Symbol: KLK1
• Synonyms: KLK1; kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein-1; Klk6; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kidney/pancreas/salivary gland kallikrein; hK1; KLKR
• Gene ID: 3816
• mRNA Refseq: NM_002257
• Protein Refseq: NP_002248
• UniProt ID: P06870