Recombinant Rat Jam2 protein(Met1-Asn236), His-tagged

• Cat.No.: Jam2-7493R
• Product Overview: Recombinant Rat JAM2 (Q3MHC0) (Met1-Asn236) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-236 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When cells (8 x 10^4 cells/well) are added to rat JAM2-His coated plates (5 μg/mL, 100 μL/well), >30% will adhere after 60 minutes at 37°C.
• Molecular Mass: The recombinant rat JAM2 comprises 219 amino acids and predicts a molecular mass of 24.7 kDa. The apparent molecular mass of the recombinant protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Jam2 junctional adhesion molecule 2 [ Rattus norvegicus ]
• Official Symbol: Jam2
• Synonyms: JAM2; junctional adhesion molecule 2; junctional adhesion molecule B; junction adhesion molecule 2
• Gene ID: 619374
• mRNA Refseq: NM_001034004
• Protein Refseq: NP_001029176