Recombinant Rat Interferon-γ

• Cat.No.: Ifng-11R
• Product Overview: Recombinant Rat Interferon-γ produced inE. coliis a single non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15.6 kDa.

Specification

• Cat. No.: Ifng-11R
• Description: Interferon-gamma (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Formulation: Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
• Specific Activity: Fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay is less than 0.1 ng/ml, corresponding to a specific activity of 1.0×10⁷IU/ mg.
• Endotoxin: Less than 1EU/mg of rrIFN-γ as determined by LAL method.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤20°C. Further dilutions should be made in appropriate buffered solutions.
• Storage: This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Tag: Non

Gene Information

• Gene Name: Ifng
• Synonyms: IFNG2; Ifg; IFN-g; IFN-gamma; Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; gamma interferon; Ifng; interferon gamma
• Gene ID: 25712
• mRNA Refseq: NM_138880
• Protein Refseq: NP_620235
• MIM: 147570
• UniProt ID: P01580
• Chromosome Location: 7q22
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Regulation of autophagy; T cell receptor signaling pathway; TGF-beta signaling pathway; Type I diabetes mellitus
• Function: cytokine activity; interferon-gamma receptor binding