Recombinant Rat ICOS protein(Glu 21 - Pro 145 (C137S

• Cat.No.: ICOS-0230R
• Product Overview: Recombinant Rat ICOS protein(Glu 21 - Pro 145 (C137S,C138S))(Q9R1T7-1) was expressed in HEK293, with the C-terminal fused polyhistidine tag.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 21-145 a.a.
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Rat ICOS / CD278 (C137S, C138S), His Tag at 5 ug/mL (100 uL/well) can bind Mouse B7-H2, Fc Tag with a linear range of 2-39 ng/mL.
• Molecular Mass: The protein has a calculated MW of 15.9 kDa. The protein migrates as 24-26 kDa and 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Icos inducible T-cell co-stimulator [ Rattus norvegicus ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation-inducible lymphocyte immunomediatory molecule; Ailim
• Gene ID: 64545
• mRNA Refseq: NM_022610
• Protein Refseq: NP_072132