Recombinant Mouse ICOS protein, hFc-tagged

• Cat.No.: ICOS-0231M
• Product Overview: Recombinant Mouse ICOS protein(Q9WVS0-1)(Glu21-Leu144), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Glu21-Leu144
• Tag: C-hFc
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Icos inducible T cell co-stimulator [ Mus musculus ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T cell co-stimulator; inducible T-cell costimulator; CD28-related protein 1; CD28 and CTLA-4-like protein; inducible T-cell co-stimulator; activation-inducible lymphocyte immunomediatory molecule; H4; CCLP; AILIM; CRP-1; Ly115
• Gene ID: 54167
• mRNA Refseq: NM_017480
• Protein Refseq: NP_059508