Recombinant Mouse ICOS protein, hFc-tagged

Cat.No. : ICOS-0231M
Product Overview : Recombinant Mouse ICOS protein(Q9WVS0-1)(Glu21-Leu144), fused with C-terminal hFc tag, was expressed in HEK293.
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Source : HEK293
Species : Mouse
Tag : C-hFc
Protein length : Glu21-Leu144
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Bio-activity : Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.329 μM as determined in SPR assay (Biacore T200).
Molecular Mass : The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Name Icos inducible T cell co-stimulator [ Mus musculus ]
Official Symbol ICOS
Synonyms ICOS; inducible T cell co-stimulator; inducible T-cell costimulator; CD28-related protein 1; CD28 and CTLA-4-like protein; inducible T-cell co-stimulator; activation-inducible lymphocyte immunomediatory molecule; H4; CCLP; AILIM; CRP-1; Ly115;
Gene ID 54167
mRNA Refseq NM_017480
Protein Refseq NP_059508

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