Recombinant Rat Icam1 protein(Met1-Thr493), His-tagged

• Cat.No.: Icam1-4086R
• Product Overview: Recombinant Rat ICAM1 (Q00238-1) (Met 1-Thr 493) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-493 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When cells are added to rat ICAM1 coated plates (12.5 μg/mL, 100 μL/well), approximately > 30% cells will adhere specifically.
• Molecular Mass: The recombinant rat ICAM1 comprises 477 amino acids and predicts a molecular mass of 52.7 kDa. The apparent molecular mass of the rat ICAM1 is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Icam1 intercellular adhesion molecule 1 [ Rattus norvegicus ]
• Official Symbol: Icam1
• Synonyms: ICAM1; intercellular adhesion molecule 1; ICAM-1; CD54; ICAM
• Gene ID: 25464
• mRNA Refseq: NM_012967
• Protein Refseq: NP_037099