Active Recombinant Human ICAM1, Fc Chimera

• Cat.No.: ICAM1-471H
• Product Overview: Recombinant Human ICAM1 (Accession # CAA30051) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Predicted N Terminal: No results obtained: Gln28 predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When 5 x 10^4 cells/well are added to recombinant human ICAM Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >60% will adhere after PMA 1 hour incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human ICAM1, Fc Chimera has a calculated MW of 76 kDa (monomer). In SDS-PAGE migrates as 110-125 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ICAM1 intercellular adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: ICAM1
• Synonyms: ICAM1; intercellular adhesion molecule 1; BB2; CD54; human rhinovirus receptor; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; P3.58
• Gene ID: 3383
• mRNA Refseq: NM_000201
• Protein Refseq: NP_000192
• MIM: 147840
• UniProt ID: P05362
• Chromosome Location: 19p13.3-p13.2