Recombinant Rat Gpi protein, His & T7-tagged

• Cat.No.: Gpi-723R
• Product Overview: Recombinant Rat Gpi aa. (Thr335~Asp558 (Accession # Q6P6V0)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the glucose phosphate isomerase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor.
• Source: E. coli
• Species: Rat
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.4kDa
• Protein length: Thr335~Asp558
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Gpi glucose-6-phosphate isomerase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gpi
• Synonyms: Gpi; glucose-6-phosphate isomerase; Amf; Nlk; Pgi; Phi; Gpi1; glucose-6-phosphate isomerase; autocrine motility factor
• Gene ID: 292804
• mRNA Refseq: NM_207592.1
• Protein Refseq: NP_997475.1
• UniProt ID: Q6P6V0