Recombinant Rat Gja5 protein, His-tagged

• Cat.No.: Gja5-6995R
• Product Overview: Recombinant Rat Gja5 aa. (Ala50~Glu303) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Ala50~Glu303
• Description: Forms intercellular connoxin channels in gap junctions; mediates cell-cell communication.
• Form: Freeze-dried powder
• Molecular Mass: 20&16kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.9.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gja5 gap junction protein, alpha 5 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Gja5
• Synonyms: Gja5; gap junction protein, alpha 5; Cx40; gap junction alpha-5 protein; connexin 40; gap junction membrane channel protein alpha 5
• Gene ID: 50563
• mRNA Refseq: NM_019280.1
• Protein Refseq: NP_062153.1
• UniProt ID: P28234