Recombinant human GDNF family receptor alpha 1 protein, Fc tagged

• Cat.No.: GFRA1-09H
• Product Overview: Recombinant human GFR alpha-1 (25-424aa), fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 25-424aa
• Description: This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Tag: C-Fc
• Form: Liquid
• Bio-activity: Measured by its binding ability in a functional ELISA with Human GDNF. The ED50 range ≤ 50 ng/mL.
• Molecular Mass: 70.8 kDa
• AA Sequence: DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• References: 1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117-126.
  2. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801-5806.
  3. Jing S, et al. (1996). Cell. 85:1113-1124.
  4. Treanor JJ, et al. (1996). 382: 80-83.
  5. Jing S, et al. (1997). J Biol Chem. 272: 33111-33117.

Gene Information

• Gene Name: GFRA1 GDNF family receptor alpha 1 [ Homo sapiens (human) ]
• Official Symbol: GFRA1
• Synonyms: GFRA1; GDNF family receptor alpha 1; GDNFR; RET1L; RETL1; RHDA4; TRNR1; GDNFRA; GFRalpha-1; GFR-ALPHA-1; GDNFR-alpha-1; GDNF family receptor alpha-1; GDNF receptor alpha 1d; GDNF receptor alpha 1e; GPI-linked anchor protein; Glial cell line-derived neurotrophic factor receptor alpha; PI-linked cell-surface accessory protein; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1
• Gene ID: 2674
• mRNA Refseq: NM_145793
• Protein Refseq: NP_665736
• MIM: 601496
• UniProt ID: P56159