Recombinant Rat Erbb2 Protein, None-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Erbb2-4099RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Rat Erbb2 (AAH61863.1) extracellular domain (Met 4-Thr 656), fused with seven additional amino acids (ALEVLFQ) at the C-terminus, was produced in Human Cell.

Specification

• Species: Rat
• Source: HEK293
• Tag: LEVLFQ
• Protein Length: 638
• Form: Lyophilized
• Molecular Mass: The recombinant rat ErbB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 638 amino acids and predicts a molecular mass of 70.6 kDa. The apparent molecular mass of the rat ErbB2 is approximately 95-105 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Erbb2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Rattus norvegicus ]
• Official Symbol: Erbb2
• Gene ID: 24337
• mRNA Refseq: NM_017003
• Protein Refseq: NP_058699

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.