Recombinant Rat Erbb2 Protein, His-tagged

• Cat.No.: Erbb2-2120R
• Product Overview: Recombinant rat Erbb2 protein, fused to His-tagged at N-terminus, was expressed in E. coli

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Form: Lyophilized from a 0.2 µM filtered solution of 20 mM Tris,150 mM NaCl, 4M Urea, pH 8.0
• Molecular Mass: 29.3kD
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: Erbb2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Rattus norvegicus ]
• Official Symbol: Erbb2
• Gene ID: 24337
• mRNA Refseq: NM_017003
• Protein Refseq: NP_058699

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.