Recombinant Rat Erbb2 protein, His & GST-tagged

• Cat.No.: Erbb2-1896R
• Product Overview: Recombinant Rat Erbb2 aa. (Phe380-Gly582 (Accession # Q8K3F9)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: GST&His
• Protein Length: Phe380-Gly582
• Description: May play a role in cell proliferation and differentiation.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 50.0kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Erbb2 erb-b2 receptor tyrosine kinase 2 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Erbb2
• Synonyms: Slit1; slit guidance ligand 1; MEGF4; slit homolog 1 protein; multiple EGF-like domains protein 4; multiple epidermal growth factor-like domains 4; multiple epidermal growth factor-like domains protein 4; slit-1
• Gene ID: 24337
• mRNA Refseq: NM_017003.2
• Protein Refseq: NP_058699.2
• UniProt ID: Q8K3F9

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.