Recombinant Rat Dld protein, His & T7-tagged

• Cat.No.: Dld-5631R
• Product Overview: Recombinant Rat Dld aa. (Asp238~Glu466 (Accession # Q6P6R2)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His&T7
• Protein Length: Asp238~Glu466
• Description: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 28.0kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Dld dihydrolipoamide dehydrogenase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Dld
• Synonyms: Dld; dihydrolipoamide dehydrogenase; dihydrolipoyl dehydrogenase, mitochondrial; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
• Gene ID: 298942
• mRNA Refseq: NM_199385.2
• Protein Refseq: NP_955417.1
• UniProt ID: Q6P6R2