Recombinant Rat Dio2 protein, His & MBP-tagged

• Cat.No.: Dio2-8147R
• Product Overview: Recombinant Rat Dio2 aa. (Ser30~Phe259 (Accession# P70551)) fused with N-terminal His & MBP tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His&MBP
• Protein Length: Ser30~Phe259
• Description: The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is expressed in a few tissues in rat, including brain, pituitary and brown adipose tissue. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. Studies in rat suggest that this gene may play an important role in the regulation of lipid metabolism and thermogenesis, and in osteoarthritis pathogenesis by enhancing the chondrocyte hypertrophy and inflammatory response. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal.
• Form: Freeze-dried powder
• Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.05% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Dio2 deiodinase, iodothyronine, type II [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Dio2
• Synonyms: 5DII; DIOII; type II iodothyronine deiodinase; deiodinase, iodothyronine, type 2; type 2 DI; type-II 5'-deiodinase
• Gene ID: 65162
• mRNA Refseq: NM_031720.4
• Protein Refseq: NP_113908.3
• UniProt ID: P70551