Recombinant Rat Cystatin C, His-tagged

• Cat.No.: Cst3-253R
• Product Overview: Recombinant Rat CaBP9K (His-tagged) produced inE. Colicontains 134 amino acids and has a molecular mass of 14.93 kDa.

Specification

• Cat. No.: Cst3-253R
• Description: Cystatins are a superfamily of cysteine proteinase inhibitors found in both plants and animals. They comprise a group of proteinase inhibitors, widely distributed in tissues and body fluids, and form tight complexes with cysteine proteases such as cathepsin B, H, L and S. Cystatin C, a secreted molecule of this family, is of interest from biochemical, medicine and evolutionary points of view. Cystatin C, with molecular weight of 13260 Da, is composed of 120 amino acids, lacks carbohydrate and has two disulfide bridges located near the carboxyl terminus. Cystatin C is increased in patients with malignant diseases, and is related to the insufficiency of renal function and appears to be a better marker than creatinine. On the other hand, low levels of cystatin C involve cause the breakdown of the elastic laminae and, subsequently, the atherosclerosis and abdominal aortic aneurysm.
• Source: Escherichia Coli.
• Amino Acid Sequence: MRGSHHHHHH GMASGTSRPP PRLLGAPQEA DASEEGVQRA LDFAVSEYNK GS NDAYHSRA IQVVRARKQLVAGINYYLDV EMGRTTCTKS QTNLTNCPFH DQP HL MR KAL CSFQIYSVPW KGTHTLTKSS.
• Formulation: Filtered (0.4 micron) and lyophilized from 0.5 mg/ml in 0.03 M Acetate buffer, pH 4.
• Purity: Greater than 96% as determined by: Analysis by reducing and non-reducing SDS-PAGE Coomassie.
• Reconstitution: Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 g/ml. In higher concentrations the solubility of this antigen is limited.
• Stability/Shelf Life: Lyophilized CABP9K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP9K should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Applications: Western blotting.
• Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Gene Information

• Gene Name: Cst3 cystatin C [ Rattus norvegicus ]
• Synonyms: Cst3; cystatin C; CYSC; MGC105556; cystatin 3; Cystatin C (cysteine proteinase inhibitor)
• Gene ID: 25307
• mRNA Refseq: NM_012837
• Protein Refseq: NP_036969
• UniProt ID: P14841
• Chromosome Location: 3q41
• Function: beta-amyloid binding; cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding; protein binding