Recombinant Mouse Cst3, His-tagged

• Cat.No.: Cst3-3669M
• Product Overview: Total 134 AA. MW: 15 kDa (calculated). N-terminal His-tag, 14 extra AA (highlighted).

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Cystatin C is mainly used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. It is a potent inhibitor of lysosomal proteinases and probably one of the most important extracellular inhibitors of cysteine proteases. Cystatin C belongs to the type 2 cystatin gene family.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM Tris buffer, 50 mM NaCl, pH 7.5.
• Research topic: Renal disease; Animal studies
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MRGSHHHHHH GMASATPKQG PRMLGAPEEA DANEEGVRRA LDFAVSEYNK GSNDAYHSRA IQVVRARKQL VAGVNYFLDV EMGRTTCTKS QTNLTDCPFH DQPHLMRKAL CSFQIYSVPW KGTHSLTKFS CKNA
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Pathways: Salivary secretion

Gene Information

• Gene Name: Cst3 cystatin C [ Mus musculus ]
• Official Symbol: Cst3
• Synonyms: Cst3; cystatin C; CysC; cystatin-3; OTTMUSP00000016736; cystatin-C; cystatin 3
• Gene ID: 13010
• mRNA Refseq: NM_009976
• Protein Refseq: NP_034106
• UniProt ID: P21460
• Chromosome Location: 2 G3; 2 84.0 cM
• Function: beta-amyloid binding; cysteine-type endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding