Recombinant Rat Cntf Protein, His-tagged

• Cat.No.: Cntf-374R
• Product Overview: Recombinant rat CNTF, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Description: CNTF also known as ciliary neurotrphic factor is a survival factor for various neuronal cell types. CNTF is highly conserved across species and exhibits cross-species bioactivity. CNTF is a 25 kDa polypeptide that was originally isolated as a target-derived survival factor of parasympathetic ciliary ganglion neurons. Binding of CNTF to CNTF receptor triggers heterodimerization of gp130 and LIFR, forming an active trimeric receptor complex and activates the downstream signaling pathway.
• Form: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 25.2 kDa (223aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Cntf ciliary neurotrophic factor [ Rattus norvegicus ]
• Official Symbol: Cntf
• Synonyms: CNTF; ciliary neurotrophic factor; ciliary neurotropic factor
• Gene ID: 25707
• mRNA Refseq: NM_013166
• Protein Refseq: NP_037298
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; growth factor activity; interleukin-6 receptor binding; interleukin-6 receptor binding