Recombinant Rat Cfd protein, His-tagged

• Cat.No.: Cfd-1860R
• Product Overview: Recombinant Rat Cfd aa. (Met1~Ala263) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His
• Protein Length: Met1~Ala263
• Description: Serine protease adipocyte-specific protein mediated by the Notch/Hes-1 signaling pathway; can be used as a fecal biomarker for abnormal intestinal epithelial differentiation.
• Form: Freeze-dried powder
• Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: Cfd complement factor D [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cfd
• Synonyms: CYP1A2; cytochrome P450 family 1 subfamily A member 2; CP12; CYPIA2; P3-450; P450(PA); cytochrome P450 1A2; P450 form 4; aryl hydrocarbon hydroxylase; cholesterol 25-hydroxylase; cytochrome P(3)450; cytochrome P450 4; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase
• Gene ID: 54249
• mRNA Refseq: NM_001077642.1
• Protein Refseq: NP_001071110.1
• UniProt ID: P32038