Recombinant Rat Cd274 protein, His & GST-tagged

• Cat.No.: Cd274-7693R
• Product Overview: Recombinant Rat Cd274 aa. (Ser34~Val241) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: 64kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser34~Val241
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.2.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Cd274 CD274 molecule [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cd274
• Synonyms: Pdcd1lg1; RGD1566211; programmed cell death 1 ligand 1
• Gene ID: 499342
• mRNA Refseq: NM_001191954.1
• Protein Refseq: NP_001178883.1
• UniProt ID: D4AE25