Recombinant Rat Bckdk protein, His & T7-tagged

• Cat.No.: Bckdk-1689R
• Product Overview: Recombinant Rat Bckdk aa. (Arg44~Thr223 (Accession # Q00972)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Mitochondrial kinase; involved in oxidative degradation of branched-chain amino acids.
• Source: E. coli
• Species: Rat
• Tag: His&T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 24.5kDa
• Protein length: Arg44~Thr223
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9

Gene Information

• Gene Name: Bckdk branched chain ketoacid dehydrogenase kinase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Bckdk
• Synonyms: Bckdk; branched chain ketoacid dehydrogenase kinase; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; BCKD-kinase; BCKDHKIN; branched-chain alpha-ketoacid dehydrogenase kinase
• Gene ID: 29603
• mRNA Refseq: NM_019244.2
• Protein Refseq: NP_062117.2
• UniProt ID: Q00972