Recombinant Rat Apaf1 Protein, His-tagged

• Cat.No.: Apaf1-197R
• Product Overview: Recombinant Rat Apaf1 Protein (Lys4-Gln344) with N-His tag was expressed in E. coli.

Specification

• Description: Enables heat shock protein binding activity and identical protein binding activity. Involved in several processes, including animal organ development; cardiac muscle cell apoptotic process; and cellular response to transforming growth factor beta stimulus. Located in cytoplasm. Part of apoptosome. Used to study visual epilepsy. Biomarker of brain ischemia; colon cancer; middle cerebral artery infarction; and muscular atrophy. Orthologous to human APAF1 (apoptotic peptidase activating factor 1).
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 38.1 kDa
• Protein length: Lys4-Gln344
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Apaf1 apoptotic peptidase activating factor 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Apaf1
• Synonyms: APAF1; apoptotic peptidase activating factor 1; apoptotic protease-activating factor 1; apaf-1; apoptotic protease activating factor 1
• Gene ID: 78963
• mRNA Refseq: NM_023979
• Protein Refseq: NP_076469
• UniProt ID: Q9EPV5