Recombinant Rabbit IgG protein, His-tagged

• Cat.No.: IgG-433R
• Product Overview: Recombinant Rabbit IgG aa. (Met1~Cys238) fused with His tag was produced in HEK293F cells.

Specification

• Source: HEK293F
• Species: Rabbit
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.0kDa; Accurate Molecular Mass: 25/55kDa as determined by SDS-PAGE reducing conditions. Rabbit IgG consists of 1455 amino acids and has a predicted molecular mass of 160 kDa. As a result of disulfide bond, the apparent molecular mass of IgG is approximately two lines 55kDa heavy chain and two lines 25kDa light chain in SDS-PAGE under reducing conditions
• Protein length: Met1~Cys238
• Endotoxin: <1.0EU per 1μg (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 4.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex