Recombinant Porcine Interleukin 6

• Cat.No.: IL6-85P
• Product Overview: Recombinant Porcine Interleukin-6 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,113 Dalton. The IL-6 is purified by proprietary chromatographic techniques.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• Description: Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Arg-Leu-Glu.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized containing no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin 6 in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀as determined by the dose-dependant stimulation of B9 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 10⁷IU/mg.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.581 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
• Stability: Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL6 should be stored at 4℃between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Sus scrofa ]
• Synonyms: interleukin 6 (interferon, beta 2); IL6; interleukin-6 protein; interleukin 6
• Gene ID: 399500
• mRNA Refseq: NM_214399
• Protein Refseq: NP_999564
• UniProt ID: P26893
• Chromosome Location: 9q14-q15
• Pathway: Cytokine-cytokine receptor interaction; Graft-versus-host disease; Hematopoietic cell lineage; Hypertrophic cardiomyopathy (HCM); Jak-STAT signaling pathway; Pathways in cancer; Prion diseases; Toll-like receptor signaling pathway