Recombinant Porcine Interleukin 10

• Cat.No.: IL10-79P
• Product Overview: Recombinant Porcine Interleukin-10 produced inE.coliis a single, glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18458 Dalton. The IL-10 is purified by proprietary chromatographic techniques.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• Description: IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Ile-Lys-Ser.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized with no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin 10 in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀as determined by the dose-dependant stimulation of MC/9 cell which was found to be < 15 ng/ml.
• Protein Content: Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 0.36 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-10 as a Reference Standard.
• Stability: Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL10 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IL10 interleukin 10 [ Sus scrofa ]
• Synonyms: interleukin 10; IL10; IL-10
• Gene ID: 397106
• mRNA Refseq: NM_214041
• Protein Refseq: NP_999206
• UniProt ID: Q29055
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway