Recombinant Porcine Interleukin-1 alpha

• Cat.No.: IL1α-81P
• Product Overview: Recombinant Porcine Interleukin-1 produced inE.Coliis single, a non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 18076 Dalton. The IL-1 is purified by proprietary chromatographic techniques.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• Description: Interleukin-1 alpha is a proinflammatory cytokine produced by a wide variety of cell types, including macrophages, osteoblasts, monocytes and hepatocytes. Circulating levels of are normally low and only rise after stimulation by agents such as those produced byinflammation, infection or microbial endotoxins. IL-1 alpha possesses a wide variety of biological activities and exerts its effects by binding to specific cell surface receptors.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Thr-Tyr-Ser.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-1 alpha in sterile 18 MΩ-cm H₂O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀as determined by the dose-dependant stimulation of D10S cells is < 0.03 ng/ml.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.669 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 as a Reference Standard.
• Stability: Lyophilized Interleukin-1 alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL1 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Pathways: Apoptosis; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Hematopoietic cell lineage; Leishmaniasis; MAPK signaling pathway; Prion diseases; Type I diabetes mellitus

Gene Information

• Gene Name: IL1A interleukin 1, alpha [ Sus scrofa ]
• Synonyms: IL1A; interleukin 1, alpha; IL-1alpha; interleukin-1 alpha; IL-1 alpha; interleukin 1-alpha
• Gene ID: 397094
• mRNA Refseq: NM_214029
• Protein Refseq: NP_999194
• UniProt ID: P18430
• Chromosome Location: 3q12-q13; 3