Recombinant Pig TNF Protein, His-tagged

• Cat.No.: TNF-436P
• Product Overview: Recombinant Pig TNF Protein (Gly57-Leu232) with N-His tag was expressed in E. coli.

Specification

• Description: Biased expression in spleen (RPKM 8.4), lung (RPKM 3.7) and 1 other tissue
• Source: E. coli
• Species: Pig
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 22.9 kDa
  Accurate Molecular Mass: 23 kDa
• Protein length: Gly57-Leu232
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Sus scrofa (pig) ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor-necrosis factor; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; TNFa
• Gene ID: 397086
• mRNA Refseq: NM_214022
• Protein Refseq: NP_999187
• UniProt ID: P23563