Recombinant Pig TNF protein, His-tagged

• Cat.No.: TNF-563P
• Product Overview: Recombinant Pig TNF aa. (Leu77~Leu232) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Pig
• Source: E.coli
• Tag: His
• Protein Length: Leu77~Leu232
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: TNF tumor necrosis factor [ Sus scrofa (pig) ]
• Official Symbol: TNF
• Synonyms: TNF; TNFa; tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
• Gene ID: 397086
• mRNA Refseq: NM_214022.1
• Protein Refseq: NP_999187.1
• UniProt ID: P23563