Recombinant Pig PCBP1 Protein, His-tagged

• Cat.No.: PCBP1-1172P
• Product Overview: Recombinant Pig PCBP1 Protein (Met1-Ser356) with His tag was expressed in E. coli.

Specification

• Description: This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
• Source: E. coli
• Species: Pig
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 41.2 kDa; Accurate Molecular Mass: 43 kDa
• Protein length: Met1-Ser356
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: PCBP1 poly(rC) binding protein 1 [ Sus scrofa (pig) ]
• Official Symbol: PCBP1
• Synonyms: PCBP1; poly(rC) binding protein 1; poly(rC)-binding protein 1
• Gene ID: 100511501
• mRNA Refseq: XM_003125057
• Protein Refseq: XP_003125105
• UniProt ID: I3LEC2