Recombinant Pig EGF protein, His-tagged

• Cat.No.: EGF-2815P
• Product Overview: Recombinant Pig EGF aa. (Ala45~His275) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Pig
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ala45~His275
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 5.8.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: EGF epidermal growth factor [ Sus scrofa (pig) ]
• Official Symbol: EGF
• Synonyms: pro-epidermal growth factor
• Gene ID: 397083
• mRNA Refseq: NM_214020.1
• Protein Refseq: NP_999185.1
• UniProt ID: Q00968